Department Of Higher Education

Department Of Higher Education has published Tender For Purchase Of Chemicals , Purchase Of Chemicals , Bamhi Size: 50000 Units Bamhi Is A Type Ii Restriction Endonuclease That Recognizes The Palindromic Dna Sequence 5′-Ggatcc-3′ And Cleaves Within This Recognition Site. It Cuts Between The First Two Guanine Residues (G^Gatcc), Generating Cohesive 5′ Overhangs (Sticky Ends). This Property Makes Bamhi Widely Used In Molecular Cloning For Precise Dna Fragment Insertion And Plasmid Construction. The Enzyme Requires Mg²⁺ As A Cofactor And Functions Optimally Under Recommended Buffer Conditions At 37 °C. Bamhi Can Be Heat-Inactivated (Commonly At 65 °C For 20 Minutes, Depending On The Supplier) And Is Typically Supplied In Glycerol For Storage At −20 °C. It Is Also Important To Maintain Optimal Reaction Conditions To Avoid Star Activity, Which Can Lead To Non-Specific Cleavage. , Onetaq® Quick-Load® 2X Master Mix With Standard Buffer Size: 500 Reactions Onetaq Quick-Load 2X Master Mix With Standard Buffer Is A Ready-To-Use Pcr Mix Containing A Blend Of Taq Dna Polymerase And A Proofreading Enzyme, Along With Dntps, Mg²⁺, And Optimized Buffer. Supplied At 2X Concentration, It Requires Only Template Dna, Primers, And Water. The Quick-Load Formulation Includes Loading Dyes, Allowing Direct Gel Loading After Pcr. It Performs Efficiently Under Standard Pcr Conditions (Extension At 68-72 °C) And Is Suitable For Routine Dna Amplification. The Mix Should Be Stored At -20 °C. Last date of submission for this tender is 2026-07-31. This is a Chemical Supply tender in Coimbatore, Tamil Nadu