Shri Mata Vaishno Devi University

Shri Mata Vaishno Devi University has published Bids Are invited for Exome Sequencing - Exome SequencingHuman Whole Exome sequencing should be carried out DNA library preparation, enrichment capture of exonic ; whole exome with UTR.Cluster amplification, paired end run to obtain 2x150bp reads with at least 100X mean depth coverage.Minimum 10-12 GB cleaned data/sample (adapter and low quality sequences/reads removed) should be delivered. Proportion of clean raw data should be 90% and above. >85% of total data should have >Q30 Phred score.Bioinformatics:-Detailed quality report before and after trimming should be provided. It should include base quality and sequence quality score, distribution details, average base content and GC distribution in the reads, PCR amplification details, check for overrepresented sequences, adaptor trimming details, read length details (percentage of read length distribution), parameters used for trimming and details of software used for trimming/removal of low quality sequences/reads etc.Alignment and recalibration, variant calling and variant annotation using appropriate software. Details of read alignment statistics and quality metrics obtained for each sample, and list of software’s and parameters should be providedDepth of coverage details, percentage and details of target regions captured and also not captured regions of the target region should be provided for each sample.Variant calling (SNP and INDELS) should be performed using standard procedures of GATK .Data will be provided though Link or hard disk. Total Quantity : 1. Last date of submission for this tender is 2021-12-25. This is a Laboratory Equipment tender in null, Jammu And Kashmir