Department Of Agricultural Research And Education

Department Of Agricultural Research And Education has published E-Tender Notice Inviting Tender For Small Rna Sequencing Of Wheat Samples As Per Tender Document , Contract For Small Rna Sequencing Of Wheat Samples , Specifications For Small Rna Sequencing Of Wheat Samples1 . A Total Of 24 Samples Of Wheat Tissuesto Be Processed 2 . Quality Control Of Samples Should Be Done Using Qubit& Agilent Bioanalyzer , Rin ( Rna Integrity Number ) And Values Should Be At Least 7-8 . 3 . Required Mrna Library Preparation Of Wheat Samples Using Illumina Platform With 1X75 Single End Sequencing Chemistry . 4 . A Total Of 20-25 Million Reads ( Or More ) Of >Q30 . Need To Generate 1-2Gb Data Per Sample . 5 . Carry Out Bioinformatics Analysis For These Samples Which Includes:A ) Quality Checking Of Sequence Reads , Filtering Of High-Quality Sequence Data And Assembly Of Contigs . Following Quality Checks Will Be Performed ( I ) Base Quality Score Distribution , ( Ii ) Sequence Quality Score Distribution , ( Iii ) Average Base Content Per Read , ( Iv ) Read Length Distribution , ( V ) Biasing Of K-Mers . B ) Small Rna Assembly And Annotation ( Mirna , Sirna , Snorna , Pirnaetc ) , Target Prediction . Raw Data With Quality And Statistical Analysis . C ) Reporting Of Known Small Rna ( Mirna And Other Non Coding Rnas ) . Prediction Of Novel Small Rnas . Prediction Of Mirna/Small Rna Targets , Mirna Based Ssr Markers Alongwith Primers List , Tasi-Mirnas Etc . D ) Mirna Network/Hub Genes For Salt Stress , Mirna Target Plot And Heat Map Of Differentially Expressed Genes . E ) If Data Does Not Fulfil The Above Criterion , Data Will Not Be Accepted . Sample Details: 24 Samples Will Be Sent To The Firm In Split ( I . E . 12 Samples In Current Financial Year And 12 Samples In The Next Year . It Will Be The Responsibility Of The Service Provider To Collect The Samples From The Lab And Transport To The Sequencing Centre Under Proper Conditions At Its Own Cost . The Institute Will Not Bear Any Cost For Trasport Of Samples . . Last date of submission for this tender is 2020-02-27. This is a Paint Supply tender in Icar-iiwbr Karnal, Haryana